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AT5G55300.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
nucleus 1.000
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:24134884 (2013): cytoskeleton microtubules
  • PMID:23396599 (2013): nucleus nuclear envelope nuclear inner membrane
  • PMID:18433157 (2008): cytosol
  • PMID:18433157 (2008): nucleus
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : DNA topoisomerase I alpha
Curator
Summary (TAIR10)
Encodes a type-I DNA topoisomerase I. Disruptions in this gene affect phyllotaxis and plant architecture suggesting that the gene plays a critical role in the maintenance of a regular pattern of organ initiation. Isolated as a protein oxidized during seed germination; proteomics approach revealed differences in de novo synthesis levels of this protein in condition with vs. without salicylic acid in the period from 0 to 40 hrs. following seed imbibition. Functions in stem cell maintenance at all stages of shoot and floral meristems and in the regulation of gene silencing.
Computational
Description (TAIR10)
DNA topoisomerase I alpha (TOP1ALPHA); FUNCTIONS IN: protein binding, DNA topoisomerase type I activity; INVOLVED IN: in 12 processes; LOCATED IN: chromosome; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DNA topoisomerase I, DNA binding, mixed alpha/beta motif, eukaryotic-type (InterPro:IPR013030), DNA topoisomerase I, DNA binding, eukaryotic-type (InterPro:IPR008336), DNA topoisomerase I, C-terminal (InterPro:IPR001631), DNA topoisomerase I, active site (InterPro:IPR018521), DNA topoisomerase I, catalytic core, alpha/beta subdomain, eukaryotic-type (InterPro:IPR014727), DNA topoisomerase I, catalytic core, eukaryotic-type (InterPro:IPR013500), DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-type (InterPro:IPR014711), DNA topoisomerase I, C-terminal, eukaryotic-type (InterPro:IPR013499), DNA breaking-rejoining enzyme, catalytic core (InterPro:IPR011010); BEST Arabidopsis thaliana protein match is: DNA topoisomerase 1 beta (TAIR:AT5G55310.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT5G55300-MONOMERBioCyc:ARA:GQT-376-MONOMERBioGrid:20867BRENDA:5.99.1.2EC:5.99.1.2eggNOG:COG3569eggNOG:KOG0981
EMBL:AB015479EMBL:CP002688EMBL:X57544EnsemblPlants:AT5G55300EnsemblPlants:AT5G55300.1entrez:835623ExpressionAtlas:P30181
Gene3D:1.10.10.41Gene3D:1.10.132.10Gene3D:2.170.11.10Gene3D:3.90.15.10GeneID:835623Genevisible:P30181GO:GO:0003677
GO:GO:0003917GO:GO:0003918GO:GO:0005634GO:GO:0005694GO:GO:0006265hmmpanther:PTHR10290hmmpanther:PTHR10290:SF3
HOGENOM:HOG000105469InParanoid:P30181IntAct:P30181InterPro:IPR001631InterPro:IPR008336InterPro:IPR011010InterPro:IPR013030
InterPro:IPR013034InterPro:IPR013499InterPro:IPR013500InterPro:IPR014711InterPro:IPR014727InterPro:IPR018521InterPro:IPR025834
iPTMnet:P30181ncoils:CoilPaxDb:P30181Pfam:P30181Pfam:PF01028Pfam:PF02919Pfam:PF14370
PhylomeDB:P30181PIR:S22864PRIDE:P30181PRINTS:PR00416PRO:PR:P30181PROSITE:PS00176ProteinModelPortal:P30181
Proteomes:UP000006548Reactome:R-ATH-4615885RefSeq:NP_200341.1scanprosite:PS00176SMART:SM00435SMR:P30181STRING:3702.AT5G55300.2
SUPFAM:SSF56349SUPFAM:SSF56741TAIR:AT5G55300tair10-symbols:MGO1tair10-symbols:TOP1tair10-symbols:TOP1ALPHAUniGene:At.197
UniProt:P30181
Coordinates (TAIR10) chr5:-:22424837..22429045
Molecular Weight (calculated) 102804.00 Da
IEP (calculated) 10.06
GRAVY (calculated) -0.90
Length 916 amino acids
Sequence (TAIR10)
(BLAST)
001: MGTETVSKPV MDNGSGDSDD DKPLAFKRNN TVASNSNQSK SNSQRSKAVP TTKVSPMRSP VTSPNGTTPS NKTSIVKSSM PSSSSKASPA KSPLRNDMPS
101: TVKDRSQLQK DQSECKIEHE DSEDDRPLSS ILSGNKGPTS SRQVSSPQPE KKNNGDRPLD RASRIIKDES DDETPISSMF RKKIDSGMSG GNQLSNDEKK
201: PLVQKLHQNG STVKNEVPNG KVLGKRPLEK NSSADQSSLK KAKISASPTS VKMKQDSVKK EIDDKGRVLV SPKMKAKQLS TREDGTDDDD DDDVPISKRF
301: KSDSSNSNTS SAKPKAVKLN STSSAAKPKA RNVVSPRSRA MTKNTKKVTK DSKYSTSSKS SPSSGDGQKK WTTLVHNGVI FPPPYKPHGI KILYKGKPVD
401: LTIEQEEVAT MFAVMRETDY YTKPQFRENF WNDWRRLLGK KHVIQKLDDC DFTPIYEWHL EEKEKKKQMS TEEKKALKEE KMKQEEKYMW AVVDGVKEKI
501: GNFRVEPPGL FRGRGEHPKM GKLKKRIHPC EITLNIGKGA PIPECPIAGE RWKEVKHDNT VTWLAFWADP INPKEFKYVF LGAGSSLKGL SDKEKYEKAR
601: NLTDHIDNIR TTYTKNFTAK DVKMRQIAVA TYLIDKLALR AGNEKDDDEA DTVGCCTLKV GNVECIPPNK IKFDFLGKDS IQYVNTVEVE PLVYKAIGQF
701: QAGKSKTDDL FDELDTSKLN AHLKELVPGL TAKVFRTYNA SITLDEMLSQ ETKDGDVTQK IVVYQKANKE VAIICNHQRT VSKTHGAQIE KLTARIEELK
801: EVLKELKTNL DRAKKGKPPL EGSDGKKIRS LEPNAWEKKI AQQSAKIEKM ERDMHTKEDL KTVALGTSKI NYLDPRITVA WCKRHEVPIE KIFTKSLLEK
901: FAWAMDVEPE YRFSRR
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)