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AT5G54160.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
cytosol 1.000
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:26781341 (2016): plasma membrane
  • PMID:26572690 (2016): extracellular region plant-type cell wall
  • PMID:25900983 (2015): Golgi Golgi apparatus Golgi membrane
  • PMID:25900983 (2015): Golgi trans-Golgi network
  • PMID:25900983 (2015): Golgi trans-Golgi network multivesicular body
  • PMID:19334764 (2009): plasma membrane
  • PMID:17317660 (2007): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : O-methyltransferase 1
Curator
Summary (TAIR10)
A caffeic acid/5-hydroxyferulic acid O-methyltransferase. Interacts with 14-4-3 proteins in yeast 2 hybrid assay. AtOMT1 (At5g54160) encodes a flavonol 3?-O-methyltransferase that is highly active towards quercetin and myricetin. The substrate specificity identifies the enzyme as flavonol 3?-methyltransferase which replaces the former annotation of the gene to encode a caffeic acid/5-hydroxyferulic acid O-methyltransferase
Computational
Description (TAIR10)
O-methyltransferase 1 (OMT1); FUNCTIONS IN: myricetin 3'-O-methyltransferase activity, quercetin 3-O-methyltransferase activity, caffeate O-methyltransferase activity; INVOLVED IN: lignin biosynthetic process, flavonol biosynthetic process; LOCATED IN: cytosol, nucleus, plasma membrane, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Winged helix-turn-helix transcription repressor DNA-binding (InterPro:IPR011991), Plant methyltransferase dimerisation (InterPro:IPR012967), O-methyltransferase, family 2 (InterPro:IPR001077), O-methyltransferase, COMT, eukaryota (InterPro:IPR016461); BEST Arabidopsis thaliana protein match is: O-methyltransferase family protein (TAIR:AT1G77520.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Protein Annotations
BioGrid:20748BRENDA:2.1.1.42BRENDA:2.1.1.76EC:2.1.1.42EC:2.1.1.68eggNOG:ENOG410XS7TeggNOG:KOG3178
EMBL:AB013387EMBL:AY062837EMBL:AY081565EMBL:AY087297EMBL:CP002688EMBL:U70424EMBL:Z27062
EnsemblPlants:AT5G54160EnsemblPlants:AT5G54160.1entrez:835504Gene3D:1.10.10.10Gene3D:3.40.50.150GeneID:835504Genevisible:Q9FK25
GO:GO:0005634GO:GO:0005737GO:GO:0005886GO:GO:0009506GO:GO:0009809GO:GO:0030744GO:GO:0030755
GO:GO:0033799GO:GO:0047763GO:GO:0051555Gramene:AT5G54160.1gramene_pathway:2.1.1.-gramene_pathway:2.1.1.68gramene_pathway:2.1.1.76
gramene_pathway:PWY-1121gramene_pathway:PWY-2181gramene_pathway:PWY-5868gramene_pathway:PWY-6064gramene_pathway:PWY-6199hmmpanther:PTHR11746hmmpanther:PTHR11746:SF106
HOGENOM:HOG000238276IntAct:Q9FK25InterPro:IPR001077InterPro:IPR011991InterPro:IPR012967InterPro:IPR016461InterPro:IPR029063
iPTMnet:Q9FK25KEGG:ath:AT5G54160KO:K13066MINT:MINT-8066865OMA:ISINARPPaxDb:Q9FK25PDB:1NII
PDBsum:1NIIPfam:PF00891Pfam:PF08100Pfam:Q9FK25Pfscan:PS51683PhylomeDB:Q9FK25PIRSF:PIRSF005739
PRIDE:Q9FK25PRO:PR:Q9FK25PROSITE:PS51683ProteinModelPortal:Q9FK25Proteomes:UP000006548Reactome:R-ATH-209931RefSeq:NP_200227.1
SMR:Q9FK25STRING:3702.AT5G54160.1SUPFAM:SSF46785SUPFAM:SSF53335TAIR:AT5G54160tair10-symbols:ATOMT1tair10-symbols:OMT1
UniGene:At.47593UniGene:At.72792UniGene:At.74847UniPathway:UPA00724UniProt:Q9FK25
Coordinates (TAIR10) chr5:+:21982075..21984167
Molecular Weight (calculated) 39620.20 Da
IEP (calculated) 5.81
GRAVY (calculated) -0.03
Length 363 amino acids
Sequence (TAIR10)
(BLAST)
001: MGSTAETQLT PVQVTDDEAA LFAMQLASAS VLPMALKSAL ELDLLEIMAK NGSPMSPTEI ASKLPTKNPE APVMLDRILR LLTSYSVLTC SNRKLSGDGV
101: ERIYGLGPVC KYLTKNEDGV SIAALCLMNQ DKVLMESWYH LKDAILDGGI PFNKAYGMSA FEYHGTDPRF NKVFNNGMSN HSTITMKKIL ETYKGFEGLT
201: SLVDVGGGIG ATLKMIVSKY PNLKGINFDL PHVIEDAPSH PGIEHVGGDM FVSVPKGDAI FMKWICHDWS DEHCVKFLKN CYESLPEDGK VILAECILPE
301: TPDSSLSTKQ VVHVDCIMLA HNPGGKERTE KEFEALAKAS GFKGIKVVCD AFGVNLIELL KKL
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)