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AT5G28540.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
endoplasmic reticulum 1.000
ASURE: endoplasmic reticulum
What is SUBAcon?
Predictors External Curations
AmiGO : endoplasmic reticulum 11402207
SwissProt : endoplasmic reticulum 16381842
TAIR : plastid 15028209
TAIR : vacuole 15539469
TAIR : plasma membrane 17151019
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:23624714 (2013): endoplasmic reticulum
  • PMID:18931141 (2008): endoplasmic reticulum
  • PMID:32219438 (2020): cytosol
  • PMID:31871212 (2020): mitochondrion
  • PMID:31615849 (2019): plastid plastid thylakoid
  • PMID:31520498 (2020): mitochondrion
  • PMID:31023727 (2019): mitochondrion
  • PMID:30962257 (2019): plastid
  • PMID:30961429 (2019): nucleus
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:30447334 (2019): plasma membrane
  • PMID:29104584 (2017): nucleus nuclear matrix nucleolus
  • PMID:28865150 (2017): extracellular region plant-type cell wall
  • PMID:27943495 (2017): mitochondrion
  • PMID:27137770 (2016): plastid plastid stroma
  • PMID:26781341 (2016): plasma membrane
  • PMID:25900983 (2015): plant-type vacuole plant-type vacuole membrane
  • PMID:25900983 (2015): Golgi trans-Golgi network multivesicular body
  • PMID:25900983 (2015): Golgi trans-Golgi network early endosome
  • PMID:25900983 (2015): Golgi
  • PMID:25900983 (2015): Golgi trans-Golgi network
  • PMID:25900983 (2015): Golgi Golgi apparatus Golgi membrane
  • PMID:24361574 (2014): plastid plastid stroma
  • PMID:23903016 (2013): plant-type vacuole plant-type vacuole membrane
  • PMID:23673981 (2013): plastid plastid stroma plastoglobules
  • PMID:23396599 (2013): nucleus nuclear envelope nuclear inner membrane
  • PMID:22923678 (2012): endoplasmic reticulum
  • PMID:22574745 (2012): mitochondrion
  • PMID:22550958 (2012): plastid
  • PMID:22522809 (2012): cytosol cytosolic ribosomes
  • PMID:22430844 (2012): Golgi
  • PMID:21988472 (2012): plant-type vacuole plant-type vacuole membrane
  • PMID:21433285 (2011): plasma membrane
  • PMID:21166475 (2011): cytosol
  • PMID:20061580 (2010): plastid plastid stroma
  • PMID:20061580 (2010): plastid plastid envelope
  • PMID:19334764 (2009): plasma membrane
  • PMID:17317660 (2007): plasma membrane
  • PMID:17151019 (2007): plant-type vacuole
  • PMID:15157177 (2004): extracellular region
  • PMID:15028209 (2004): plastid
  • PMID:14505352 (2003): nucleus
FP Images

Arabidopsis cell culture (peroxisomal marker)

At5g-GFP-28540
(full-length)
RFP-peroxisomal markeroverlay

Images by John Bussel

onion epidermal cell layer (peroxisomal marker)

At5g-GFP-28540
(full-length)
RFP-peroxisomal markeroverlay

Images by John Bussel
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : heat shock protein 70 (Hsp 70) family protein
Curator
Summary (TAIR10)
Encodes the luminal binding protein BiP, an ER-localized member of the HSP70 family. BiP is composed of an N-terminal ATP binding domain and a C-terminal domain that binds to hydrophobic patches on improperly/incompletely folded proteins in an ATP-dependent manner. Involved in polar nuclei fusion during proliferation of endosperm nuclei.
Computational
Description (TAIR10)
BIP1; FUNCTIONS IN: ATP binding; INVOLVED IN: protein folding, ER-associated protein catabolic process, response to heat, polar nucleus fusion; LOCATED IN: cell wall, plasma membrane, chloroplast, vacuole, endoplasmic reticulum lumen; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, seedling growth; CONTAINS InterPro DOMAIN/s: Heat shock protein 70, conserved site (InterPro:IPR018181), Heat shock protein Hsp70 (InterPro:IPR001023), Heat shock protein 70 (InterPro:IPR013126); BEST Arabidopsis thaliana protein match is: Heat shock protein 70 (Hsp 70) family protein (TAIR:AT5G42020.1); Has 36391 Blast hits to 35786 proteins in 4820 species: Archae - 162; Bacteria - 17493; Metazoa - 3988; Fungi - 1814; Plants - 1283; Viruses - 341; Other Eukaryotes - 11310 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:GQT-238-MONOMERBioGrid:18223eggNOG:COG0443eggNOG:KOG0100
EMBL:AF262043EMBL:AK221441EMBL:BT000453EMBL:BT001212
EMBL:CP002688EMBL:D89341EMBL:Z17760EnsemblPlants:AT5G28540
EnsemblPlants:AT5G28540.1entrez:832950Gene3D:1.20.1270.10Gene3D:2.60.34.10
GeneID:832950Genevisible:Q9LKR3GO:GO:0005524GO:GO:0005618
GO:GO:0005773GO:GO:0005774GO:GO:0005783GO:GO:0005788
GO:GO:0005794GO:GO:0005829GO:GO:0005886GO:GO:0006351
GO:GO:0006355GO:GO:0009506GO:GO:0009507GO:GO:0016592
GO:GO:0030433Gramene:AT5G28540.1hmmpanther:PTHR19375hmmpanther:PTHR19375:SF219
HOGENOM:HOG000228135InParanoid:Q9LKR3IntAct:Q9LKR3InterPro:IPR013126
InterPro:IPR018181InterPro:IPR029047InterPro:IPR029048iPTMnet:Q9LKR3
KEGG:ath:AT5G28540KO:K09490MINT:MINT-7950246ncoils:Coil
OMA:ANIAHIRPaxDb:Q9LKR3Pfam:PF00012Pfam:Q9LKR3
Pfscan:PS51257PhylomeDB:Q9LKR3PRIDE:Q9LKR3PRINTS:PR00301
PRO:PR:Q9LKR3PROSITE:PS00014PROSITE:PS00297PROSITE:PS00329
PROSITE:PS01036ProteinModelPortal:Q9LKR3Proteomes:UP000006548RefSeq:NP_198206.1
scanprosite:PS00297scanprosite:PS00329scanprosite:PS01036SMR:Q9LKR3
STRING:3702.AT5G28540.1SUPFAM:SSF100920SUPFAM:SSF100934SUPFAM:SSF53067
SWISS-2DPAGE:Q9LKR3TAIR:AT5G28540tair10-symbols:BIP1TMHMM:TMhelix
UniGene:At.23381UniGene:At.49118UniGene:At.49188UniGene:At.64764
UniProt:Q9LKR3
Coordinates (TAIR10) chr5:-:10540665..10543274
Molecular Weight (calculated) 73633.40 Da
IEP (calculated) 4.81
GRAVY (calculated) -0.46
Length 669 amino acids
Sequence (TAIR10)
(BLAST)
001: MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD
101: VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN
201: VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER
301: AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV
401: AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLNGIPPAPR
501: GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE
601: KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GGAGGESSTE EEDESHDEL
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)