AT4G22970.1
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Subcellular Consensus
(Prediction and Experimental) min:  :max.
SUBAcon:nucleus 1.000 What is SUBAcon? |
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| Experimental Localisations and PPI |
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SUBAcon links
AGI-AGI relationships |
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| Description (TAIR10) | protein_coding : homolog of separase | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Curator Summary (TAIR10) |
Encodes a separase (ESP), homologous to human and mouse separase protein. Separase is a capase family protease required for the release of sister chromatid cohesion during meiosis and mitosis. Arabidopsis separase contains a predicted 2Fe2S-ferredoxin domain that is not present in the proteins of other organisms. Also contains a putative EF-hand calcium binding domain. Mutant seeds exhibited embryo arrest at the globular stage. The endosperm also exhibited a weak titan-like phenotype. Transgenic plants expressing AESP RNA interference (RNAi) from the meiosis-specific DMC1 promoter exhibited alterations in chromosome segregation during meiosis I and II that resulted in polyads containing from one to eight microspores. Plays an essential role in embryo development. Required for the removal of cohesin from meiotic chromosomes and establishment of meiotic nuclear domains. This gene was also identified through the rsw4 mutant. Lines carrying recessive, temperature-sensitive mutations exhibit reduced anisotropic growth at 30 degrees Celsius. Microtubules and cellulose microfibrils are not depleted or disoriented in the mutants at the restrictive temperature. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Computational Description (TAIR10) |
homolog of separase (ESP); FUNCTIONS IN: peptidase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase C50, separase (InterPro:IPR005314); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28550.1); Has 401 Blast hits to 372 proteins in 177 species: Archae - 0; Bacteria - 2; Metazoa - 98; Fungi - 137; Plants - 87; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Protein Annotations |
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| Coordinates (TAIR10) | chr4:-:12033703..12043572 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Molecular Weight (calculated) | 244860.00 Da | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| IEP (calculated) | 7.14 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GRAVY (calculated) | -0.19 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Length | 2180 amino acids | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sequence (TAIR10) (BLAST) |
0001: MASSGDDLRL LSLIDVGDNV FSSFSDYLKP FSTLSTSRKK QDRATTIRAL AKQFLPFLNK SISLLPKRLS VANSDKEARE SALDLFRAYE LCLDCLELVS 0101: AQLACKPHTV QSQRLRMIHC LDVWGLYENV YTEAFKVLEK LRGSDSKSRK SRLLPEVQDG DAEMALVVVD AVAAIFRAVA MSQQLDDKRY RKVLLLLEEV 0201: GGWLRVLDAK VYEKLHRAMV TSMGKCAVSL VREAERFNGD LVISFCDLTV KEHYKSALSK DRVYKFAREV LSVLFGFKDR KMSVTIDISM SVLRSLSCQF 0301: EDESNENLME FFDLVDYCAH KFRAAGDMYC AKVSKKLNEM AAIFVEAIPQ LNLVLRLYST GLSITVCNSK LGEIKLEDST DDWKIQAMFD DDARWQSLVS 0401: LLGMVDSYSG DEGNQTGSSS IGGHRNYNNK THDSCKDRNK ITCWPQYVDA LKFLCQPLAD FIYSVKRKIV LETEMSCASA HLITIHDAFL QFCDGCLFLQ 0501: RCTSDKGDRE IANNKAFLNA AMGAFIVSLR TQLKLEISAH LVEDVIGSPW IQSQELKYLI ATLYNIGIVL YRNKELNKAC EALKLCSKVS WRCVELHCHM 0601: FVNQSSSSDN DLSEDAIMDF VGEACNRCAF YLDILQKCSR RKIRQNIVHI LENWLSAEHL IRRLPGPEAI VKQWVKIERE CHTDLDAAGS CTTLYSLLSS 0701: SQKKSKRGIG KILEQELLAY DRVLPLRSNL GQQTRIKIAD ILLKDVYVTE DMHIERARIL IWKARMTRTS GTEHITECIC FLSEAISILG ELHHGPNEEG 0801: SPSSHMLPIA YCLRAFCTQE ADPNSKKVFQ DISTSLNLWL RILSLDDSGD SLPTENIIPL LYNMIDLMSV KGCTELHHHI YQLIFRLFKW KNVKLEVCLA 0901: MLWECRRLSH ALCPSPISDA FIQTLSENCA DKSTCIDFWM DCLKDSKAKL IGFQQNFHDL HNKDEGPFQS DITIDDIKDA ASELISSASL SGNSSFAAAY 1001: LYYDLCERLI SFGKLSEALS YAKEAYRIRT LIFQDKFKYT AEKHIEKHNE DGKISEIRTF SIKNFQVYRL LATDFWPCGN FLWDINRCYL SPWSVLQCYL 1101: ESTLQVGILN ELIGNGLEAE TILSWGKAFS CSQSLFPFVV AFSSALGNLY HKKQCLDLAE KELQNAKEIL IANQRDFSCV KCKLKLEVTL DKQLGDISRK 1201: QIDRVSQTDG FLHAESLFSA ALGKFCCSAW KSCIRSHGEE IAEEIVIDRN GGEGLGHNSS KTKLSIKEPP GNRGSRRGGR ANKTCLSKDQ DLISEPTSRL 1301: TRSMRHSLRE QCQNRSNVPE VVSKKPNLCD RSVGSRGERV LLDTSNALPG FCICYKEKRQ QCLSEEVTES GSLNNLVSLK WELCHRKLAS SILVSLGKCL 1401: GDSGRIHLAH EALLHSISVL FKSTWSSHNQ PSVSQLLEFI GKEVTRDVFA VDRAIILYNL CWLNLRNYHC RKSRSICCDL FHIPFTKLVS WLMLAFVLSG 1501: EVPILFQKVS RLLASLYLLS SSNSEFTFES DGNELSASHW VSFFHQASLG THLSYHFISN LSQKHKSQCL SDKECTEATC SSCMVPEDLD LPRLAPDRTQ 1601: DLVQFAKEFF INLPSSTIIC ISLLGGALNQ LLQELMHIRS PVCAWVLISR LNPESQPVAT LLPVDSIVED MSDDSANLSS TEATQVKSLK GPWLCPWGTT 1701: VVDEVAPAFK SILEESHSSS STTEEDTIES RGLWWKKRKK LNHRLGIFLR NLEASWLGPW RCLLLGEWSN YKLPDSAQKK LVNDLKSKCK MEVNEMLLKV 1801: ILGGGTDNFK GEACVAQLSL RNGCYVGRGG YLYEEDSCKT PTAASNISES RHELALKLIH DAASKLGQQD GHENREPIIL VLDPEVQMLP WENIPILRKQ 1901: EVYRMPSVGC ISAVLKKRSL QGEPAKSHVA SFPLIDPLDS FYLLNPGGDL TDTQVTFESW FRDQNFEGKA GSEPSAIELT EALETHDLFL YFGHGSGAQY 2001: IPRREIEKLD NCSATFLMGC SSGSLWLKGC YIPQGVPLSY LLGGSPAIVA TLWDVTDRDI DRFGKALLEA WLQERSDSSS EGGCSQCESL ANDLAAMTLK 2101: GTKRSRKPSS RNKPAQSDVD GSGKIECNHK HRRKIGSFIA AARDACNLQY LIGAAPVCYG VPTGITRKKG IDALLPSSSR |
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| See Also |
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Citation
If you find this resource useful please cite one of the following publications:
Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)
Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)