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AT3G44320.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
cytosol 0.497
peroxisome 0.283
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31975158 (2020): plastid
  • PMID:31871212 (2020): mitochondrion
  • PMID:31520498 (2020): mitochondrion
  • PMID:30962257 (2019): plastid
  • PMID:30783145 (2019): extracellular region plant-type cell wall
  • PMID:29104584 (2017): nucleus nuclear matrix nucleolus
  • PMID:28865150 (2017): extracellular region plant-type cell wall
  • PMID:26572690 (2016): extracellular region plant-type cell wall
  • PMID:24361574 (2014): plastid plastid stroma
  • PMID:23396599 (2013): nucleus nuclear envelope nuclear inner membrane
  • PMID:21988472 (2012): plant-type vacuole plant-type vacuole membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : nitrilase 3
Curator
Summary (TAIR10)
This enzyme catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA) (EC 3.5.5.1) and IAN to indole-3-acetamide (IAM) at lower levels. It is the only one of the four Arabidopsis nitrilases whose mRNA levels are strongly induced when plants experience sulphur deprivation. This enzyme likely participates in other non-auxin-related metabolic pathways.
Computational
Description (TAIR10)
nitrilase 3 (NIT3); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: cellular response to sulfate starvation, response to cadmium ion, glucosinolate catabolic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 1 (TAIR:AT3G44310.3); Has 7365 Blast hits to 7291 proteins in 1693 species: Archae - 150; Bacteria - 4836; Metazoa - 437; Fungi - 564; Plants - 330; Viruses - 11; Other Eukaryotes - 1037 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT3G44320-MONOMERBioCyc:MetaCyc:AT3G44320-MONOMERBioGrid:8877BRENDA:3.5.5.1
EC:3.5.5.1eggNOG:COG0388eggNOG:KOG0805EMBL:AL353865
EMBL:BT002773EMBL:BT004345EMBL:CP002686EMBL:U09959
EMBL:Y07648EnsemblPlants:AT3G44320EnsemblPlants:AT3G44320.1entrez:823557
Gene3D:3.60.110.10GeneID:823557Genevisible:P46010GO:GO:0000257
GO:GO:0005886GO:GO:0006807GO:GO:0009970GO:GO:0019762
GO:GO:0046686GO:GO:0080061GO:GO:0080109Gramene:AT3G44320.1
gramene_plant_reactome:1119486gramene_plant_reactome:6876740hmmpanther:PTHR23088hmmpanther:PTHR23088:SF31
HOGENOM:HOG000256365InParanoid:P46010InterPro:IPR000132InterPro:IPR003010
KEGG:ath:AT3G44320KO:K01501MINT:MINT-8065341OMA:ACERLIW
PaxDb:P46010Pfam:P46010Pfam:PF00795Pfscan:PS50263
PhylomeDB:P46010PIR:T49148PRIDE:P46010PRO:PR:P46010
PROSITE:PS00920PROSITE:PS00921PROSITE:PS50263ProteinModelPortal:P46010
Proteomes:UP000006548RefSeq:NP_190018.1scanprosite:PS00920scanprosite:PS00921
SMR:P46010STRING:3702.AT3G44320.1SUPFAM:SSF56317TAIR:AT3G44320
tair10-symbols:AtNIT3tair10-symbols:NIT3UniGene:At.242UniProt:P46010
Coordinates (TAIR10) chr3:+:15993419..15995493
Molecular Weight (calculated) 38024.30 Da
IEP (calculated) 5.81
GRAVY (calculated) -0.21
Length 346 amino acids
Sequence (TAIR10)
(BLAST)
001: MSSTEEMSSV KNTTQVIGVD PSSTVRVTIV QSSTVYNDTP ATLDKAEKFI VEAASKGAKL VLFPEAFIGG YPRGFRFGLA VGVHNEEGRD EFRNYHASAI
101: KVPGPEVERL AELAGKNNVH LVMGAIEKDG YTLYCTALFF SPQGQFLGKH RKVMPTSLER CIWGQGDGST IPVYDTPIGK IGAAICWENR MPLYRTALYA
201: KGIEIYCAPT ADYSLEWQAS MIHIAVEGGC FVLSAHQFCK RREFPEHPDY LFNDIVDTKE HDPTVSGGGS VIISPLGKVL AGPNYESEGL VTADLDLGDI
301: ARAKLYFDVV GHYSKPDIFN LTVNEHPKKP VTFMTKVEKA EDESNK
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)