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AT2G39930.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
plastid 1.000
ASURE: plastid
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : isoamylase 1
Curator
Summary (TAIR10)
Encodes an isoamylase-type debranching enzyme. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. Mutants have reduced starch content and abnormally structured amylopectins and phytoglycogens. It has been postulated that AtISA1 interacts with AtISA2 to form the Iso1 complex.
Computational
Description (TAIR10)
isoamylase 1 (ISA1); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, amylopectin biosynthetic process; LOCATED IN: chloroplast, chloroplast isoamylase complex; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Immunoglobulin-like fold (InterPro:IPR013783), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 17979 Blast hits to 17918 proteins in 2410 species: Archae - 161; Bacteria - 15130; Metazoa - 257; Fungi - 410; Plants - 747; Viruses - 0; Other Eukaryotes - 1274 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT2G39930-MONOMERBioGrid:3918BRENDA:3.2.1.68CAZy:CBM48CAZy:GH13EC:3.2.1.68eggNOG:COG1523
eggNOG:ENOG410IN7NEMBL:AF002109EMBL:BT000443EMBL:BT010348EMBL:CP002685EnsemblPlants:AT2G39930EnsemblPlants:AT2G39930.1
entrez:818580Gene3D:2.60.40.10Gene3D:2.60.40.1180Gene3D:3.20.20.80GeneID:818580Genevisible:O04196GO:GO:0005977
GO:GO:0009507GO:GO:0010021GO:GO:0010368GO:GO:0019156GO:GO:0019252Gramene:AT2G39930.1hmmpanther:PTHR10357
hmmpanther:PTHR10357:SF169HOGENOM:HOG000239197InParanoid:O04196IntAct:O04196InterPro:IPR004193InterPro:IPR006047InterPro:IPR013780
InterPro:IPR013781InterPro:IPR013783InterPro:IPR014756InterPro:IPR015902InterPro:IPR017853KEGG:ath:AT2G39930KO:K01214
OMA:YAQDNPIPANTHER:PTHR10357PaxDb:O04196Pfam:O04196Pfam:PF00128Pfam:PF02922PhylomeDB:O04196
PIR:B84823PRIDE:O04196PRO:PR:O04196ProteinModelPortal:O04196Proteomes:UP000006548RefSeq:NP_181522.1SMART:SM00642
SMR:O04196STRING:3702.AT2G39930.1SUPFAM:SSF51011SUPFAM:SSF51445SUPFAM:SSF81296TAIR:AT2G39930tair10-symbols:ATISA1
tair10-symbols:ISA1UniGene:At.20831UniGene:At.71018UniPathway:UPA00152UniProt:O04196
Coordinates (TAIR10) chr2:+:16666078..16672183
Molecular Weight (calculated) 89485.70 Da
IEP (calculated) 6.12
GRAVY (calculated) -0.38
Length 783 amino acids
Sequence (TAIR10)
(BLAST)
001: MDAIKCSSSF LHHTKLNTLF SNHTFPKISA PNFKPLFRPI SISAKDRRSN EAENIAVVEK PLKSDRFFIS DGLPSPFGPT VRDDGVNFSV YSTNSVSATI
101: CLISLSDLRQ NKVTEEIQLD PSRNRTGHVW HVFLRGDFKD MLYGYRFDGK FSPEEGHYYD SSNILLDPYA KAIISRDEFG VLGPDDNCWP QMACMVPTRE
201: EEFDWEGDMH LKLPQKDLVI YEMHVRGFTR HESSKIEFPG TYQGVAEKLD HLKELGINCI ELMPCHEFNE LEYYSYNTIL GDHRVNFWGY STIGFFSPMI
301: RYASASSNNF AGRAINEFKI LVKEAHKRGI EVIMDVVLNH TAEGNEKGPI FSFRGVDNSV YYMLAPKGEF YNYSGCGNTF NCNHPVVRQF ILDCLRYWVT
401: EMHVDGFRFD LGSIMSRSSS LWDAANVYGA DVEGDLLTTG TPISCPPVID MISNDPILRG VKLIAEAWDA GGLYQVGMFP HWGIWSEWNG KFRDVVRQFI
501: KGTDGFSGAF AECLCGSPNL YQGGRKPWHS INFICAHDGF TLADLVTYNN KNNLANGEEN NDGENHNYSW NCGEEGDFAS ISVKRLRKRQ MRNFFVSLMV
601: SQGVPMIYMG DEYGHTKGGN NNTYCHDNYM NYFRWDKKEE AHSDFFRFCR ILIKFRDECE SLGLNDFPTA KRLQWHGLAP EIPNWSETSR FVAFSLVDSV
701: KKEIYVAFNT SHLATLVSLP NRPGYRWEPF VDTSKPSPYD CITPDLPERE TAMKQYRHFL DANVYPMLSY SSIILLLSPI KDP
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)