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AT2G25600.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

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SUBAcon:
plasma membrane 1.000
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:31541795 (2020): plasma membrane
  • PMID:30875866 (2019): plasma membrane
  • PMID:25402197 (2015): plastid plastid thylakoid plastid thylakoid membrane
  • PMID:22318864 (2012): plasma membrane
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : Shaker pollen inward K+ channel
Curator
Summary (TAIR10)
Encodes SPIK, a member of the Shaker family potassium ion (K+) channel. This family includes five groups based on phylogenetic analysis (FEBS Letters (2007) 581: 2357): I (inward rectifying channel): AKT1 (AT2G26650), AKT5 (AT4G32500) and SPIK (also known as AKT6, AT2G25600); II (inward rectifying channel): KAT1 (AT5G46240) and KAT2 (AT4G18290); III (weakly inward rectifying channel): AKT2 (AT4G22200); IV (regulatory subunit involved in inwardly rectifying conductance formation): KAT3 (also known as AtKC1, AT4G32650); V (outward rectifying channel): SKOR (AT3G02850) and GORK (AT5G37500). Mutant plants have impaired pollen-tube growth.
Computational
Description (TAIR10)
Shaker pollen inward K+ channel (SPIK); FUNCTIONS IN: inward rectifier potassium channel activity, cyclic nucleotide binding, potassium channel activity; INVOLVED IN: pollen tube growth; LOCATED IN: membrane; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Cyclic nucleotide-binding (InterPro:IPR000595), Potassium channel, voltage-dependent, EAG/ELK/ERG (InterPro:IPR003938), Protein of unknown function DUF3354 (InterPro:IPR021789), Ankyrin repeat-containing domain (InterPro:IPR020683), Ion transport (InterPro:IPR005821), Cyclic nucleotide-binding-like (InterPro:IPR018490), RmlC-like jelly roll fold (InterPro:IPR014710), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: K+ transporter 5 (TAIR:AT4G32500.1); Has 83148 Blast hits to 34215 proteins in 1581 species: Archae - 129; Bacteria - 8355; Metazoa - 40552; Fungi - 7952; Plants - 3977; Viruses - 1178; Other Eukaryotes - 21005 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:GQT-2555-MONOMERBioGrid:2451eggNOG:ENOG410XPSEeggNOG:KOG0498
EMBL:AC006053EMBL:AJ309323EMBL:AK118279EMBL:CP002685
EnsemblPlants:AT2G25600EnsemblPlants:AT2G25600.1entrez:817099Gene3D:1.25.40.20
Gene3D:2.60.120.10GeneID:817099Genevisible:Q8GXE6GO:GO:0005249
GO:GO:0016021Gramene:AT2G25600.1hmmpanther:PTHR10217hmmpanther:PTHR10217:SF504
HOGENOM:HOG000238230InParanoid:Q8GXE6InterPro:IPR000595InterPro:IPR002110
InterPro:IPR003938InterPro:IPR005821InterPro:IPR014710InterPro:IPR018490
InterPro:IPR020683InterPro:IPR021789KEGG:ath:AT2G25600OMA:HESIQGY
PaxDb:Q8GXE6Pfam:PF00027Pfam:PF00520Pfam:PF11834
Pfam:PF12796Pfam:PF13857Pfam:Q8GXE6Pfscan:PS50042
Pfscan:PS50088Pfscan:PS50297Pfscan:PS51490PhylomeDB:Q8GXE6
PIR:D84650PRIDE:Q8GXE6PRINTS:PR01463PRO:PR:Q8GXE6
PROSITE:PS50042PROSITE:PS50088PROSITE:PS50297PROSITE:PS51490
ProteinModelPortal:Q8GXE6Proteomes:UP000006548Reactome:R-ATH-1296072RefSeq:NP_180131.3
SMART:SM00100SMART:SM00248SMR:Q8GXE6STRING:3702.AT2G25600.1
SUPFAM:SSF48403SUPFAM:SSF51206SUPFAM:SSF81324TAIR:AT2G25600
tair10-symbols:AKT6tair10-symbols:SPIKTCDB:1.A.1.4.8TMHMM:TMhelix
UniGene:At.39001UniProt:Q8GXE6
Coordinates (TAIR10) chr2:+:10894603..10898369
Molecular Weight (calculated) 99220.40 Da
IEP (calculated) 6.77
GRAVY (calculated) -0.12
Length 888 amino acids
Sequence (TAIR10)
(BLAST)
001: MEKKKVWFWG VKDDGEGGGG RGGGRTKDAE DDVADHLSRD GTMSQYSLSK GLLPSLGANN RSSRDVILPR FIVSPFDPRY RAWETFLVFL VLYTAWASPF
101: EFGFLQKPRP PLSILDNIVN GFFAVDIVLT FFVAFLDKVT YLLVDDPKRI AWRYASTWLI FDVVSTFPYE IFGSLLHESI QGYGIFSMLR LWRLRRVSNC
201: FARLEKDRKY SYFWVRCSKL LLVTLFVIHC GACFLYSIAA HYPDPSKTFM ALTDENWKES PIAVRYNTAM YWSITTFSTT GYGDIHGVNS REMTFILFYM
301: VFNLGLSAYI IGNMTNLVVH VTGRTRKFRD TIQAASGFGQ RNNLPVRLQD QMVAHLCLRY RTDSEGLQQQ EIIDSLPKAI RSSISHYLFY EVVDKIYLFH
401: GISNDLLFQL VTEMKAEYFP PKEDVILQNE APTDFYILVT GAVDIIARVN GVEQVVSEAQ RGHVFGEVGV LCYRPQLFTV RTKRLSQLLR LNRTVLLNLV
501: QANVGDGAII MNNLLQHLKD SEDPVMKGVL ADTEHMLAQG KMDLPLSLCF AAARGDDLLL HQLLRRGSSP NEMDKDGRTA LHIAASKGSH YCVVLLLEHG
601: ADPNIRDSEG NVPLWEAIIG RHREIAKLLA ENGAKLSLDS VSYFSGLAVE KNCLDALKDI IKYGGDVTLP DGNGTTALHR AVSEGHLEIV KFLLDQGADL
701: DWPDSYGWTP RGLADHQGNE EIKTLFHNHR PVEKKPKPIP GIPQSPVTGK PLMKYSSEPT MHSGELVLDG GQVVVSQKRK LNNFRNSLFG IISAANSADD
801: GGEVPRSPAV PGGGGSMIYP ERVTISSPEN GETGGKVVLL PNSMEELLKI GENKMGFVPT KVLTREGAEI DDITLIRDGD FLLLSRDP
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)