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AT1G60490.1
Subcellular Consensus
(Prediction and Experimental)
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SUBAcon:
vacuole 0.957
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
  • PMID:23667806 (2013): plastid plastid thylakoid
SUBAcon links
AGI-AGI relationships
Coexpression PPI
Description (TAIR10) protein_coding : vacuolar protein sorting 34
Curator
Summary (TAIR10)
Encodes a phosphatidylinositol 3-kinase that is expressed in most plant tissues. Defects in VPS34 affect a number of cellular processes. Loss of function mutations are not transmitted through the male gametophyte due to defects in microgametogenesis therefore it is difficult to assess the effects of loss of VPS34 function in the whole plant. Involved in salt-stress responses.
Computational
Description (TAIR10)
vacuolar protein sorting 34 (VPS34); FUNCTIONS IN: 1-phosphatidylinositol-3-kinase activity, inositol or phosphatidylinositol kinase activity, binding, phosphotransferase activity, alcohol group as acceptor; INVOLVED IN: oxygen and reactive oxygen species metabolic process, response to salt stress, endocytosis, N-terminal protein myristoylation, microgametogenesis; LOCATED IN: phosphoinositide 3-kinase complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Phosphatidylinositol 3-/4-kinase, catalytic (InterPro:IPR000403), Phosphoinositide 3-kinase, accessory (PIK) domain (InterPro:IPR001263), Phosphoinositide 3-kinase, C2 (InterPro:IPR002420), C2 calcium/lipid-binding domain, CaLB (InterPro:IPR008973), Protein kinase-like domain (InterPro:IPR011009), Phosphatidylinositol Kinase (InterPro:IPR015433), Phosphatidylinositol 3-kinase, Vps34 type (InterPro:IPR008290), Armadillo-type fold (InterPro:IPR016024), Phosphatidylinositol 3/4-kinase, conserved site (InterPro:IPR018936); BEST Arabidopsis thaliana protein match is: Phosphatidylinositol 3- and 4-kinase family protein (TAIR:AT1G49340.1); Has 3550 Blast hits to 3392 proteins in 278 species: Archae - 0; Bacteria - 0; Metazoa - 1514; Fungi - 819; Plants - 335; Viruses - 4; Other Eukaryotes - 878 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT1G60490-MONOMERBioGrid:27568BRENDA:2.7.1.137EC:2.7.1.137
eggNOG:COG5032eggNOG:KOG0906EMBL:AC002292EMBL:BT000878
EMBL:CP002684EMBL:U10669EnsemblPlants:AT1G60490EnsemblPlants:AT1G60490.1
entrez:842344Gene3D:1.10.1070.11Gene3D:1.25.40.70Gene3D:2.60.40.150
GeneID:842344Genevisible:P42339GO:GO:0000045GO:GO:0000407
GO:GO:0005524GO:GO:0005768GO:GO:0005777GO:GO:0006468
GO:GO:0006897GO:GO:0009651GO:GO:0016197GO:GO:0016303
GO:GO:0030242GO:GO:0034271GO:GO:0034272GO:GO:0036092
GO:GO:0048015GO:GO:0055046GO:GO:0072593Gramene:AT1G60490.1
hmmpanther:PTHR10048hmmpanther:PTHR10048:SF62HOGENOM:HOG000174003InParanoid:P42339
IntAct:P42339InterPro:IPR000008InterPro:IPR000403InterPro:IPR001263
InterPro:IPR002420InterPro:IPR011009InterPro:IPR015433InterPro:IPR016024
InterPro:IPR018936KEGG:ath:AT1G60490KO:K00914OMA:LAITVWD
PANTHER:PTHR10048PaxDb:P42339Pfam:P42339Pfam:PF00454
Pfam:PF00613Pfam:PF00792Pfscan:PS50290Pfscan:PS51545
Pfscan:PS51547PhylomeDB:P42339PIR:B96630PRIDE:P42339
PRO:PR:P42339PROSITE:PS00915PROSITE:PS00916PROSITE:PS50290
PROSITE:PS51545PROSITE:PS51547ProteinModelPortal:P42339Proteomes:UP000006548
Reactome:R-ATH-1632852Reactome:R-ATH-1660514Reactome:R-ATH-1660516Reactome:R-ATH-1660517
Reactome:R-ATH-5668599RefSeq:NP_176251.1scanprosite:PS00915scanprosite:PS00916
SMART:SM00142SMART:SM00145SMART:SM00146SMR:P42339
STRING:3702.AT1G60490.1SUPFAM:SSF48371SUPFAM:SSF49562SUPFAM:SSF56112
TAIR:AT1G60490tair10-symbols:ATVPS34tair10-symbols:PI3Ktair10-symbols:VPS34
UniGene:At.1545UniProt:P42339
Coordinates (TAIR10) chr1:-:22285792..22290190
Molecular Weight (calculated) 93332.70 Da
IEP (calculated) 6.75
GRAVY (calculated) -0.26
Length 814 amino acids
Sequence (TAIR10)
(BLAST)
001: MGANEFRFFL SCDINSPVTF RIEKLDGNLP VKKSSDSGVV SIAEEKKPEL YIECALYIDG APFGLPMRTR LKTTGPPYCW NELITLSSKY RDLTAHSQLA
101: ITVWDVSCGK TEGLIGGATV LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT STPGKVPRHE RGELERLEKL MNKYERGQIQ SIDWLDRLML KSLDTIKEQE
201: STKHGSSHLF VVIDFCSFEH RVVFQESGAN LFITAPIGST NEFVTVWDTE LGKTNPSENK QLKLARSLDR GIIDRDLKPS NIERKSIQRV LKYPPTRTLS
301: GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWEMID VCDALELLSP LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD
401: RSCLSQFLVQ RALQNIELAS FLRWYVAVEL HDHVYAKRFY STYELLEENI IKLPPGVNGE DGYQLWQSLV RQTELTAQLC SITREVRNVR GNTQKKIEKL
501: RQLLGGLLSE LTYFEEPIRS PLTPNVLIKG IVAGESSLFK SALHPLRLTF RTPEEGGSCK LIFKKGDDLR QDQLVVQMVW LMDRLLKLEN LDLCLTPYKV
601: LATGHDEGML EFIPSRSLAQ ILSEHRSITS YLQKFHPDEH APFGITATCL DTFIKSCAGY SVITYILGIG DRHLDNLLLT DDGRLFHVDF AFILGRDPKP
701: FPPPMKLCKE MVEAMGGAES QYYTRFKSYC CEAYNILRKS SNLILNLFHL MAGSTIPDIA SDPEKGILKL QEKFRLDMDD EACIHFFQDL INESVSALFP
801: QMVETIHRWA QYWR
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)