suba logo
AT3G50660.1
Subcellular Consensus
(Prediction and Experimental)
min: heatmap :max

.
SUBAcon:
endoplasmic reticulum 1.000
ASURE: endoplasmic reticulum
What is SUBAcon?
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : Cytochrome P450 superfamily protein
Curator
Summary (TAIR10)
Encodes a 22α hydroxylase whose reaction is a rate-limiting step in brassinosteroid biosynthetic pathway. The protein is a member of CYP90B gene family. CLM is an epi-allele with small, compressed rosette, reduced internode length, and reduced fertility, appears in selfed ddm mutant plants possibly due to loss of cytosine methylation. Transcripts accumulate in actively growing tissues, and GUS expression is negatively regulated by brassinosteroids. Localized in the endoplasmic reticulum. The in vitro expressed protein can perform the C-22 hydroxylation of a variety of C27-, C28- and C29-sterols. Cholesterol was the best substrate, followed by campesterol. Sitosterol was a poor substrate.
Computational
Description (TAIR10)
DWARF 4 (DWF4); CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G05690.1); Has 31120 Blast hits to 31036 proteins in 1650 species: Archae - 61; Bacteria - 5303; Metazoa - 10591; Fungi - 5722; Plants - 7996; Viruses - 3; Other Eukaryotes - 1444 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT3G50660-MONOMERBioCyc:MetaCyc:AT3G50660-MONOMERBRENDA:1.14.99.B1EC:1.14.-.-
eggNOG:COG2124eggNOG:KOG0157EMBL:AF044216EMBL:AF412114
EMBL:AL132979EMBL:AY090266EMBL:CP002686EnsemblPlants:AT3G50660
EnsemblPlants:AT3G50660.1entrez:824229Gene3D:1.10.630.10GeneID:824229
Genevisible:O64989GO:GO:0005506GO:GO:0005783GO:GO:0009741
GO:GO:0009753GO:GO:0009826GO:GO:0009867GO:GO:0010012
GO:GO:0010268GO:GO:0010358GO:GO:0016021GO:GO:0016125
GO:GO:0016132GO:GO:0016705GO:GO:0020037GO:GO:0048366
Gramene:AT3G50660.1gramene_pathway:1.14.13.-gramene_pathway:PWY-2582gramene_pathway:PWY-699
gramene_plant_reactome:1119456gramene_plant_reactome:6875096gramene_plant_reactome:6876165gramene_plant_reactome:6876432
gramene_plant_reactome:6876718hmmpanther:PTHR24286hmmpanther:PTHR24286:SF37HOGENOM:HOG000237614
InParanoid:O64989InterPro:IPR001128InterPro:IPR002401InterPro:IPR017972
KEGG:ath:AT3G50660KO:K09587ncoils:CoilOMA:LEMAIFI
PaxDb:O64989Pfam:O64989Pfam:PF00067PIR:T46143
PRIDE:O64989PRINTS:PR00385PRINTS:PR00463PRO:PR:O64989
PROSITE:PS00086ProteinModelPortal:O64989Proteomes:UP000006548Reactome:R-ATH-211916
Reactome:R-ATH-5365859RefSeq:NP_190635.1SABIO-RK:O64989scanprosite:PS00086
SMR:O64989STRING:3702.AT3G50660.1SUPFAM:SSF48264TAIR:AT3G50660
tair10-symbols:CLMtair10-symbols:CYP90B1tair10-symbols:DWF4tair10-symbols:PSC1
tair10-symbols:SAV1tair10-symbols:SNP2TMHMM:TMhelixUniGene:At.26401
UniPathway:UPA00381UniProt:O64989
Coordinates (TAIR10) chr3:-:18814262..18817168
Molecular Weight (calculated) 58871.00 Da
IEP (calculated) 7.28
GRAVY (calculated) -0.30
Length 513 amino acids
Sequence (TAIR10)
(BLAST)
001: MFETEHHTLL PLLLLPSLLS LLLFLILLKR RNRKTRFNLP PGKSGWPFLG ETIGYLKPYT ATTLGDFMQQ HVSKYGKIYR SNLFGEPTIV SADAGLNRFI
101: LQNEGRLFEC SYPRSIGGIL GKWSMLVLVG DMHRDMRSIS LNFLSHARLR TILLKDVERH TLFVLDSWQQ NSIFSAQDEA KKFTFNLMAK HIMSMDPGEE
201: ETEQLKKEYV TFMKGVVSAP LNLPGTAYHK ALQSRATILK FIERKMEERK LDIKEEDQEE EEVKTEDEAE MSKSDHVRKQ RTDDDLLGWV LKHSNLSTEQ
301: ILDLILSLLF AGHETSSVAI ALAIFFLQAC PKAVEELREE HLEIARAKKE LGESELNWDD YKKMDFTQCV INETLRLGNV VRFLHRKALK DVRYKGYDIP
401: SGWKVLPVIS AVHLDNSRYD QPNLFNPWRW QQQNNGASSS GSGSFSTWGN NYMPFGGGPR LCAGSELAKL EMAVFIHHLV LKFNWELAED DKPFAFPFVD
501: FPNGLPIRVS RIL
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)