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AT4G09020.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
plastid 1.000
ASURE: plastid
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : isoamylase 3
Curator
Summary (TAIR10)
Encodes an isoamylase-like protein. Mutant studies show that the gene is strongly involved in starch breakdown. A GUS-protein fusion product was shown to localize to the surface of chloroplastic structures reminiscent of starch granules. In the mutants, the chloroplastic α-amylase AMY3 is upregulated.
Computational
Description (TAIR10)
isoamylase 3 (ISA3); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast, chloroplast stroma, chloroplast starch grain; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl hydrolase, family 13, all-beta (InterPro:IPR013780), Immunoglobulin-like fold (InterPro:IPR013783), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 1 (TAIR:AT2G39930.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT4G09020-MONOMERBioCyc:MetaCyc:AT4G09020-MONOMERBRENDA:3.2.1.68CAZy:CBM48CAZy:GH13EC:3.2.1.68eggNOG:COG1523
eggNOG:ENOG410IN7NEMBL:AK227049EMBL:AL161513EMBL:AY091058EMBL:AY133739EMBL:CP002687EnsemblPlants:AT4G09020
EnsemblPlants:AT4G09020.1entrez:826481Gene3D:2.60.40.10Gene3D:2.60.40.1180Gene3D:3.20.20.80GeneID:826481Genevisible:Q9M0S5
GO:GO:0005977GO:GO:0005983GO:GO:0009507GO:GO:0009569GO:GO:0009570GO:GO:0019156Gramene:AT4G09020.1
hmmpanther:PTHR10357hmmpanther:PTHR10357:SF74HOGENOM:HOG000239197InParanoid:Q9M0S5InterPro:IPR004193InterPro:IPR006047InterPro:IPR013780
InterPro:IPR013781InterPro:IPR013783InterPro:IPR014756InterPro:IPR015902InterPro:IPR017853iPTMnet:Q9M0S5KEGG:ath:AT4G09020
KO:K01214OMA:GETNDLNPANTHER:PTHR10357PaxDb:Q9M0S5Pfam:PF00128Pfam:PF02922Pfam:Q9M0S5
PhylomeDB:Q9M0S5PIR:B85091PRIDE:Q9M0S5PRO:PR:Q9M0S5ProteinModelPortal:Q9M0S5Proteomes:UP000006548RefSeq:NP_192641.2
SMART:SM00642SMR:Q9M0S5STRING:3702.AT4G09020.1SUPFAM:SSF51011SUPFAM:SSF51445SUPFAM:SSF81296TAIR:AT4G09020
tair10-symbols:ATISA3tair10-symbols:ISA3UniGene:At.33717UniPathway:UPA00153UniProt:Q9M0S5
Coordinates (TAIR10) chr4:+:5784099..5788839
Molecular Weight (calculated) 86326.10 Da
IEP (calculated) 5.96
GRAVY (calculated) -0.47
Length 764 amino acids
Sequence (TAIR10)
(BLAST)
001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)