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AT1G22940.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
plastid 1.000
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : thiamin biosynthesis protein, putative
Curator
Summary (TAIR10)
Encodes a bifunctional enzyme required for thiamine (vitamin B1) biosynthesis. TH1 can phosphorylate HMP-P to produce HMP-PP, the pyrimidine heterocyclic subunit of thiamine. TH1 also catalyzes the condensation of HMP-PP and HET to form thiamine monophosphate (TMP). TH1 also appears capable of phosphorylating HMP based on E.coli mutant complementation assays. th1 mutants are thiamine auxotrophs that die as seedlings on unsupplemented media.
Computational
Description (TAIR10)
THIAMINE REQUIRING 1 (TH1); FUNCTIONS IN: phosphomethylpyrimidine kinase activity, thiamin-phosphate diphosphorylase activity, hydroxymethylpyrimidine kinase activity; INVOLVED IN: thiamin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine monophosphate synthase (InterPro:IPR003733), Phosphomethylpyrimidine kinase type-2 (InterPro:IPR004399), Aldolase-type TIM barrel (InterPro:IPR013785), Phosphomethylpyrimidine kinase type-1 (InterPro:IPR013749); Has 15989 Blast hits to 15891 proteins in 2548 species: Archae - 338; Bacteria - 12149; Metazoa - 165; Fungi - 331; Plants - 107; Viruses - 0; Other Eukaryotes - 2899 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:AT1G22940-MONOMERBioCyc:MetaCyc:AT1G22940-MONOMEREC:2.5.1.3EC:2.7.1.49eggNOG:COG0351eggNOG:COG0352eggNOG:KOG2598
EMBL:AF000657EMBL:AY128364EMBL:BT020417EMBL:CP002684EnsemblPlants:AT1G22940EnsemblPlants:AT1G22940.1entrez:838901
Gene3D:3.20.20.70Gene3D:3.40.1190.20GeneID:838901Genevisible:Q5M731GO:GO:0004789GO:GO:0005524GO:GO:0008902
GO:GO:0008972GO:GO:0009228GO:GO:0009229GO:GO:0009507GO:GO:0009570GO:GO:0046872Gramene:AT1G22940.1
gramene_pathway:2.5.1.3gramene_pathway:2.7.1.49gramene_pathway:2.7.4.7gramene_pathway:THISYNARA-PWYgramene_plant_reactome:1119629gramene_plant_reactome:6876162gramene_plant_reactome:6876720
HAMAP:MF_00097hmmpanther:PTHR20858hmmpanther:PTHR20858:SF17HOGENOM:HOG000155781InParanoid:Q5M731InterPro:IPR003733InterPro:IPR004399
InterPro:IPR013749InterPro:IPR013785InterPro:IPR022998InterPro:IPR029056KEGG:00730+2.5.1.3KEGG:00730+2.7.1.49KEGG:ath:AT1G22940
KO:K14153OMA:GINIERMPaxDb:Q5M731Pfam:PF02581Pfam:PF08543Pfam:Q5M731PhylomeDB:Q5M731
PIR:E86363PRIDE:Q5M731PRO:PR:Q5M731ProteinModelPortal:Q5M731Proteomes:UP000006548RefSeq:NP_173707.2SMR:Q5M731
STRING:3702.AT1G22940.1SUPFAM:SSF51391SUPFAM:SSF53613TAIR:AT1G22940tair10-symbols:TH-1tair10-symbols:TH1tair10-symbols:THIE
TIGRfam:TIGR00097TIGRfam:TIGR00693TIGRFAMs:TIGR00097TIGRFAMs:TIGR00693UniGene:At.43074UniGene:At.49905UniPathway:UPA00060
UniProt:Q5M731
Coordinates (TAIR10) chr1:+:8122384..8124908
Molecular Weight (calculated) 55816.10 Da
IEP (calculated) 6.51
GRAVY (calculated) 0.02
Length 522 amino acids
Sequence (TAIR10)
(BLAST)
001: MNSLGGIRSW PANWRSTTAS MTTTESVRKV PQVLTVAGSD SGAGAGIQAD LKVCAARGVY CASVITAVTA QNTRGVQSVH LLPPEFISEQ LKSVLSDFEF
101: DVVKTGMLPS TEIVEVLLQN LSDFPVRALV VDPVMVSTSG HVLAGSSILS IFRERLLPIA DIITPNVKEA SALLDGFRIE TVAEMRSAAK SLHEMGPRFV
201: LVKGGDLPDS SDSVDVYFDG KEFHELRSPR IATRNTHGTG CTLASCIAAE LAKGSSMLSA VKVAKRFVDN ALDYSKDIVI GSGMQGPFDH FFGLKKDPQS
301: SRCSIFNPDD LFLYAVTDSR MNKKWNRSIV DALKAAIEGG ATIIQLREKE AETREFLEEA KACIDICRSH GVSLLINDRI DIALACDADG VHVGQSDMPV
401: DLVRSLLGPD KIIGVSCKTP EQAHQAWKDG ADYIGSGGVF PTNTKANNRT IGLDGLKEVC EASKLPVVAI GGIGISNAGS VMQIDAPNLK GVAVVSALFD
501: QDCVLTQAKK LHKTLKESKR GI
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)