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AT1G03310.1
Subcellular Consensus
(Prediction and Experimental)

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SUBAcon:
plastid 1.000
ASURE: plastid
What is SUBAcon?
What is ASURE?
SUBAcon computations
Experimental Localisations and PPI
FP MS/MS PPI
SUBAcon links
AGI-AGI relationships
Coexpression PPI
no PPI data
Description (TAIR10) protein_coding : debranching enzyme 1
Curator
Summary (TAIR10)
Encodes a protein with strong similarity to isoamylase (EC:3.2.1.68) however lacks critical residues known to be important for activity. Appears to co localize with ISA1 in the chloroplast isoamylase complex. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. It has been postulated that AtISA2 interacts with AtISA1 to form the Iso1 complex.
Computational
Description (TAIR10)
debranching enzyme 1 (DBE1); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, amylopectin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 10041 Blast hits to 10011 proteins in 2123 species: Archae - 109; Bacteria - 8214; Metazoa - 136; Fungi - 197; Plants - 613; Viruses - 0; Other Eukaryotes - 772 (source: NCBI BLink).
Protein Annotations
BioCyc:ARA:GQT-2133-MONOMERBioCyc:ARA:GQT-2134-MONOMERBioCyc:MetaCyc:AT1G03310-MONOMERBRENDA:3.2.1.68CAZy:CBM48CAZy:GH13eggNOG:COG1523
eggNOG:ENOG410IN7NEMBL:AC005278EMBL:AY139980EMBL:CP002684EnsemblPlants:AT1G03310EnsemblPlants:AT1G03310.1EnsemblPlants:AT1G03310.2
entrez:839531Gene3D:2.60.40.10Gene3D:2.60.40.1180Gene3D:3.20.20.80GeneID:839531Genevisible:Q8L735GO:GO:0005977
GO:GO:0009507GO:GO:0010021GO:GO:0019156GO:GO:0019252Gramene:AT1G03310.1Gramene:AT1G03310.2hmmpanther:PTHR10357
hmmpanther:PTHR10357:SF115HOGENOM:HOG000239197InParanoid:Q8L735InterPro:IPR004193InterPro:IPR006047InterPro:IPR013780InterPro:IPR013781
InterPro:IPR013783InterPro:IPR014756InterPro:IPR015902InterPro:IPR017853KEGG:ath:AT1G03310OMA:IKSMKDMPANTHER:PTHR10357
PaxDb:Q8L735Pfam:PF00128Pfam:PF02922Pfam:Q8L735PhylomeDB:Q8L735PIR:F86164PRIDE:Q8L735
PRO:PR:Q8L735ProteinModelPortal:Q8L735Proteomes:UP000006548RefSeq:NP_171830.1RefSeq:NP_973751.1SMART:SM00642SMR:Q8L735
STRING:3702.AT1G03310.1SUPFAM:SSF51011SUPFAM:SSF51445SUPFAM:SSF81296TAIR:AT1G03310tair10-symbols:ATISA2tair10-symbols:BE2
tair10-symbols:DBE1tair10-symbols:ISA2UniGene:At.24041UniPathway:UPA00152UniProt:Q8L735
Coordinates (TAIR10) chr1:+:813975..816623
Molecular Weight (calculated) 98889.00 Da
IEP (calculated) 5.72
GRAVY (calculated) -0.27
Length 882 amino acids
Sequence (TAIR10)
(BLAST)
001: MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF
101: RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL
201: KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR
301: YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL
401: KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL
501: NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN
601: FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG
701: DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP
801: NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA
See Also
Citation
If you find this resource useful please cite one of the following publications:

Hooper CM, Castleden I, Tanz SK, Aryamanesh, and Millar, AH (2017) SUBA4: the interactive data analysis centre for Arabidopsis subcellular protein locations Nucleic Acids Res. Jan 4;45(D1):D1064-D1074. doi: 10.1093/nar/gkw1041 (PubMed)

Hooper CM, Tanz SK, Castleden IR, Vacher MA, Small ID, Millar AH (2014) "SUBAcon: a consensus algorithm for unifying the subcellular localization data of the Arabidopsis proteome. Bioinformatics." 1;30(23):3356-64. (Bioinformatics) (PubMed)