About SUBA3
The SubCellular Proteomic Database (SUBA3) houses large scale proteomic and GFP localisation sets from cellular compartments of Arabidopsis. It also contains precompiled bioinformatic predictions for protein subcellular localisations. A new dataset of protein-protein interactions has recently been added.
AT5G28540.1
Subcellular Localization

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SUBAcon:
endoplasmic reticulum 1.000

Gold Standard: endoplasmic reticulum
Predictors Annotations GFP MS/MS PPI GFP Images
AdaBoost : endoplasmic reticulum
ATP : no data
BaCelLo : cytosol
ChloroP : no data
EpiLoc : endoplasmic reticulum
iPSORT : endoplasmic reticulum
iPSORT : extracellular
iPSORT : golgi
MitoPred : no data
Mitoprot 2 : no data
MultiLoc : endoplasmic reticulum
Nucleo : no data
PCLR : no data
Plant-mPloc : endoplasmic reticulum
Plant-mPloc : nucleus
Predotar : endoplasmic reticulum
Predotar : extracellular
Predotar : golgi
PredSL : endoplasmic reticulum
PredSL : extracellular
PredSL : golgi
PProwler : endoplasmic reticulum
PProwler : extracellular
PProwler : golgi
PTS1 : no data
SLPFA : endoplasmic reticulum
SLPFA : extracellular
SLPFA : golgi
SLP-Local : endoplasmic reticulum
SLP-Local : extracellular
SLP-Local : golgi
SubLoc : cytosol
TargetP : endoplasmic reticulum
TargetP : extracellular
TargetP : golgi
WoLF PSORT : endoplasmic reticulum
YLoc : endoplasmic reticulum
 AmiGO : endoplasmic reticulum: 11402207
SwissProt : endoplasmic reticulum
TAIR : plastid: 15028209
TAIR : vacuole: 15539469
TAIR : plasma membrane: 17151019
 endoplasmic reticulum : 18931141
 cytosol : 22522809
cytosol : 21166475
endoplasmic reticulum : 22923678
extracellular : 15157177
golgi : 22430844
nucleus : 23396599
nucleus : 14505352
plasma membrane : 21433285
plasma membrane : 19334764
plasma membrane : 17317660
plastid : 20061580
plastid : 15028209
vacuole : 17151019
 AT3G21350.1 : 17560376

Arabidopsis cell culture (peroxisomal marker)

At5g-GFP-28540
(full-length)		RFP-peroxisomal markeroverlay

Images by John Bussel

onion epidermal cell layer (peroxisomal marker)

At5g-GFP-28540
(full-length)		RFP-peroxisomal markeroverlay

Images by John Bussel
last updated: Tue Feb 7 12:49:47 2012 v0.0.17
Description (TAIR10) protein_coding :heat shock protein 70 (Hsp 70) family protein
Curator
Summary (TAIR10)		 Encodes the luminal binding protein BiP, an ER-localized member of the HSP70 family. BiP is composed of an N-terminal ATP binding domain and a C-terminal domain that binds to hydrophobic patches on improperly/incompletely folded proteins in an ATP-dependent manner. Involved in polar nuclei fusion during proliferation of endosperm nuclei.
Computational
Description (TAIR10)		 BIP1; FUNCTIONS IN: ATP binding; INVOLVED IN: protein folding, ER-associated protein catabolic process, response to heat, polar nucleus fusion; LOCATED IN: cell wall, plasma membrane, chloroplast, vacuole, endoplasmic reticulum lumen; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, seedling growth; CONTAINS InterPro DOMAIN/s: Heat shock protein 70, conserved site (InterPro:IPR018181), Heat shock protein Hsp70 (InterPro:IPR001023), Heat shock protein 70 (InterPro:IPR013126); BEST Arabidopsis thaliana protein match is: Heat shock protein 70 (Hsp 70) family protein (TAIR:AT5G42020.1); Has 36391 Blast hits to 35786 proteins in 4820 species: Archae - 162; Bacteria - 17493; Metazoa - 3988; Fungi - 1814; Plants - 1283; Viruses - 341; Other Eukaryotes - 11310 (source: NCBI BLink).
Coordinates (TAIR10) chr5:-:10540665..10543274
Molecular Weight (calculated) 73633.4 Da
IEP (calculated) 4.81
GRAVY (calculated) -0.46
Length 668 amino acids
Sequence (TAIR10)
(BLAST) 		MARSFGAN STVVLAII FFGCLFAL SSAIEEAT KLGSVIGI DLGTTYSC VGVYKNGH VEIIANDQ GNRITPSW VGFTDSER LIGEAAKN QAAVNPER TVFDVKRL IGRKFEDK EVQKDRKL VPYQIVNK DGKPYIQV KIKDGETK VFSPEEIS AMILTKMK ETAEAYLG KKIKDAVV TVPAYFND AQRQATKD AGVIAGLN VARIINEP TAAAIAYG LDKKGGEK NILVFDLG GGTFDVSV LTIDNGVF EVLSTNGD THLGGEDF DHRVMEYF IKLIKKKH QKDISKDN KALGKLRR ECERAKRA LSSQHQVR VEIESLFD GVDFSEPL TRARFEEL NNDLFRKT MGPVKKAM DDAGLQKS QIDEIVLV GGSTRIPK VQQLLKDF FEGKEPNK GVNPDEAV AYGAAVQG GILSGEGG DETKDILL LDVAPLTL GIETVGGV MTKLIPRN TVIPTKKS QVFTTYQD QQTTVSIQ VFEGERSL TKDCRLLG KFDLNGIP PAPRGTPQ IEVTFEVD ANGILNVK AEDKASGK SEKITITN EKGRLSQE EIDRMVKE AEEFAEED KKVKEKID ARNALETY VYNMKNQV NDKDKLAD KLEGDEKE KIEAATKE ALEWLDEN QNSEKEEY DEKLKEVE AVCNPIIT AVYQRSGG APGGAGGE SSTEEEDE SHDEL
Hydropathy Plot
(raw data) 		Hydropathy plot AT5G28540.1
See Also Aramemnon AtProteome DBGET Inparanoid MIPS MPSS Plus PPDB PlantSpecDB ProMEX Proteins Wiki SALK (inserts) SALK (signal) TAIR SwissProt

Citation
If you find this resource useful please cite one of the following publications:
Tanz SK, Castleden I, Hooper CM, Vacher M, Small I; Millar, AH (2013) SUBA3: a database for integrating experimentation and prediction to define the SUBcellular location of proteins in Arabidopsis. Nucleic Acids Res. 41: D1185-91 (Nucleic Acids Res.) (PubMed)
  Heazlewood JL, Verboom RE, Tonti-Filippini J, Small I and Millar AH. (2007) SUBA: the Arabidopsis Subcellular Database. Nucleic Acids Res. 35(D):213-218. (PubMed)
  Heazlewood JL, Tonti-Filippini J, Verboom RE, Millar AH. (2005) Combining experimental and predicted datasets for determination of the subcellular location of proteins in Arabidopsis. Plant Physiol. 139(2):598-609. (PubMed)